Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries

Martínez, Lidia Dora and Noher de Halac, Inés ORCID: https://orcid.org/0000-0003-2930-9282 (1995) Organogenesis of anther-derived calluses in long-term cultures of Oenothera hookeri de Vries. Plant Cell, Tissue and Organ Culture, 42 (1). pp. 91-96. ISSN 1573-5044

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Resumen

Anthers of O. hookeri containing uninucleate microspores were cultured, in vitro, at 25°C (16 hours photoperiod) on solid MS medium. After 10-15 days, on media with 2,4-dichlorophenoxyacetic acid, 1-naphthaleneacetic acid and 6-benzylaminopurine, anthers developed friable calluses. After unsuccessful treatments on embryogenic-and/or organogenic-induction media, calluses were placed on a hormone-free MS medium for 24 months with routine transfers every 3 weeks. After this period, the calluses developed buds and subsequently plants. Ro generation plants, were morphologically distinct.

Tipo de documento:	Artículo
DOI:	https://doi.org/10.1007/BF00037686
Palabras clave:	Anther culture. Calius. Oenothera. Organogenesis.
Temas:	S Agricultura > SB Cultura de la planta
Unidad académica:	Universidad Católica de Córdoba > Facultad de Ciencias Agropecuarias
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/3716

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