Nicolas, Juan Carlos ORCID: https://orcid.org/0000-0001-9163-2668 and Grumelli, Yanina Alejandra
ORCID: https://orcid.org/0000-0001-6234-865X
(2004)
ELISA method for assess liver - Kidney microsomes antibodies
[Método ELISA para anticuerpos antimicrosomales hígado-riñón].
Acta Bioquímica Clínica Latinoamericana, 38 (1).
pp. 23-27.
ISSN 0325-2957
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Resumen
Almost twenty years ago a new type of autoimmune chronic hepatitis, denominated Type 2 Autoimmune Hepatitis was described. It is more frequent in girls and young women, characterized by the presence of serum autoantibodies liver/kidney anti microsomes type 1 (LKM-1). A microsomic enzyme of 50 kDa, the Cytochrome P450 Monooxygenase (P450IID6) metabolizes drugs and is the target antigen of the LKM-1. This antigen can also be expressed in the cellular membrane of the hepatocytes modulated by interleukines and tumoral necrosis factor (TNF). The most frequent method to determine antibodies anti LKM-1 is the indirect immunofluorescence, using liver and kidney rat slides. The main inconvenience is the similar pattern that they present with other antibodies denominated Antimitochondrial (AMA). In this work, a test for LKM is developed using an Enzime-linked Immunosorbent Assay (ELISA), starting from extracted liver rat microsomes. The Interassay Variation Coefficients for two significance levels, both negative and positive-LKM samples were respectively 12.5% and 8.7%. Sensibility and specificity were 100% and 97.5% respectively for both samples.
Tipo de documento: | Artículo |
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Palabras clave: | Autoantibodies anti liver/kidney microsomes. Autoimmune hepatitis. Enzimoimmunoassay. Immunoblot. Immunofluorescence. Microsomes. |
Temas: | Q Ciencia > QD Química |
Unidad académica: | Universidad Católica de Córdoba > Facultad de Ciencias Químicas |
Google Académico: | |
URI: | http://pa.bibdigital.ucc.edu.ar/id/eprint/3982 |
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