Rapid HCP5 single-nucleotide polymorphism genotyping: a simple allele-specific PCR method for prediction of hypersensitivity reaction to Abacavir

Galván, Cristian Ariel ORCID: https://orcid.org/0000-0002-1885-3664, Elbarcha, Osvaldo César, Fernández, Eduardo J., Beltramo, Dante Miguel ORCID: https://orcid.org/0000-0002-8198-3525 and Soria, Néstor Walter ORCID: https://orcid.org/0000-0002-8232-1822 (2011) Rapid HCP5 single-nucleotide polymorphism genotyping: a simple allele-specific PCR method for prediction of hypersensitivity reaction to Abacavir. Clinica Chimica Acta.

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Resumen

Background: The most important factor limiting the success of an antiretroviral therapy is toxicity. The HLA-B*5701 allele is predictive of hypersensitivity reaction to Abacavir, and this gene is in a perfect linkage disequilibrium with the rs2395029 SNP present in the HCP5 gene. Methods: Genomic DNA was extracted from blood obtained from 201 unrelated healthy Argentinean volunteers. The DNA was subjected to an allele-specific PCR method. Sequencing was performed to validate the test results. Results: We were successful to amplify specific fragment of interest from the DNA samples. The method is easy, specific and reproducible. Conclusions: The application of this methodology is a rapid and simple method to detect the HCP5 polymorphism (rs2395029) previous to administration of Abacavir in patients with HIV infection.

Tipo de documento:	Artículo
DOI:	https://doi.org/10.1016/j.cca.2011.04.010
Palabras clave:	5701; Abacavir hypersensitivity; Allele specific PCR; Genotyping; HCP5 polymorphism; HLA-B; Pharmacogenetics
Temas:	Q Ciencia > QD Química
Unidad académica:	Universidad Católica de Córdoba > Facultad de Ciencias Químicas
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/5420

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