Genome-wide identification of MITE-derived microRNAs and their targets in bread wheat

Crescente, Juan M., Zavallo, Diego ORCID: https://orcid.org/0000-0002-9021-2175, del Vas, Mariana ORCID: https://orcid.org/0000-0002-8365-8582, Asurmendi, Sebastián ORCID: https://orcid.org/0000-0001-9516-5948, Helguera, Marcelo ORCID: https://orcid.org/0000-0003-4401-8731, Fernández, Elmer Andrés ORCID: https://orcid.org/0000-0002-4711-8634 and Vanzetti, Leonardo S. ORCID: https://orcid.org/0000-0002-7688-5648 (2022) Genome-wide identification of MITE-derived microRNAs and their targets in bread wheat. BMC Genomics, 23 (1). ISSN 1471-2164

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Resumen

Background: Plant miRNAs are a class of small non-coding RNAs that can repress gene expression at the post-transcriptional level by targeting RNA degradation or promoting translational repression. There is increasing evidence that some miRNAs can derive from a group of non-autonomous class II transposable elements called Miniature Inverted-repeat Transposable Elements (MITEs). Results: We used public small RNA and degradome libraries from Triticum aestivum to screen for microRNAs production and predict their cleavage target sites. In parallel, we also created a comprehensive wheat MITE database by identifying novel elements and compiling known ones. When comparing both data sets, we found high homology between MITEs and 14% of all the miRNAs production sites detected. Furthermore, we show that MITE-derived miRNAs have preference for targeting degradation sites with MITE insertions in the 3’ UTR regions of the transcripts. Conclusions: Our results revealed that MITE-derived miRNAs can underlay the origin of some miRNAs and potentially shape a regulatory gene network. Since MITEs are found in millions of insertions in the wheat genome and are closely linked to genic regions, this kind of regulatory network could have a significant impact on the post-transcriptional control of gene expression.

Tipo de documento:	Artículo
DOI:	https://doi.org/10.1186/s12864-022-08364-4
Palabras clave:	miARN. ARN. Expresión génica.
Temas:	Q Ciencia > Q Ciencia (General) Q Ciencia > QK Botánica
Unidad académica:	Universidad Católica de Córdoba > Unidad Asociada a CONICET
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/3302

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