Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae

Rosa, Alberto Luis dir. ORCID: https://orcid.org/0000-0001-8701-4525 (2016) Biodiversidad genética y bioquímica de piruvato decarboxilasas en cepas indigenas de saccharomyces cerevisiae. [Proyecto de investigación]

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Resumen

Pyruvate decarboxylase (PDC) catalyzes the thiamine pyrophosphate-and magnesium-dependent decarboxylation of pyruvate to acetaldehyde and carbon dioxide, a relevant pathway in ethanol production. The budding yeast S. cerevisiae is a major player in alcoholic fermentation and a detailed knowledge of the biochemistry and metabolic control of the fermentative pathway in this microorganism is available. Our laboratory has contributed to the study of the genes encoding PDC in the filamentous fungus N. crassa (i.e. cfp-1 and cfp-2). We also discovered that PDC in filamentous fungi assembles into macromolecular filaments co-localizing with cytoplasmic microtubules. We showed that abundance and organization of PDC-filaments in fungi is dependent on the carbon sources and/or metabolic conditions of the cell. In S. cerevisiae, three structural genes for PDC have been identified (i.e. PDC1, PDC5 and PDC6) but the potential genetic and protein diversity of PDC genes in indigenous strains of S. cerevisiae has not been systematically studied. Also, the subcellular distribution and potential supramolecular arrangement of PDC from indigenous S. crerevisiae strains, perhaps during budding growth on alternative carbon sources and/or pseudohyphal growth under nutrient deprivation, has not been explored yet. In this project we will explore the molecular genetic diversity, biochemical properties and subcellular distribution of wild PDC variants from indigenous S. cerevisiae strains. Results from this project will contribute to the knowledge of the genetic and phenotypic diversity of S. cerevisiae PDC genes and their protein products. These studies also constitute the basis for future analyses of PDC genes from the isolated indigenous non-Saccharomyces yeasts strains.

Tipo de documento:	Proyecto
Información adicional:	UNIDAD ASOCIADA AL CONICET – ÁREA CIENCIAS AGRARIAS, INGENIERÍA, CIENCIAS BIOLÓGICAS Y DE LA SALUD / FACULTAD DE CIENCIAS QUÍMICAS
Palabras clave:	Uva. Levaduras. Piruvato decarboxilasa.
Temas:	Q Ciencia > QD Química Q Ciencia > QH Historia Natural > QH426 Genética
Unidad académica:	Universidad Católica de Córdoba > Facultad de Ciencias Químicas Universidad Católica de Córdoba > Unidad Asociada a CONICET
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/967

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