Evidence of bacterial Biofilms in nasal polyposis

Zernotti, Mario Emilio ORCID: https://orcid.org/0000-0003-4288-2809, Villegas, Ángel, Revol, Roque, Baena Cagnani, Carlos E., Miranda Arce, J.E., Paredes, M.E., Albesa, I. and Paraje, María Gabriela ORCID: https://orcid.org/0000-0001-8600-1159 (2010) Evidence of bacterial Biofilms in nasal polyposis. Evidence of bacterial Biofilms in nasal polyposis, 20 (5). pp. 380-385. ISSN 20945603

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Resumen

Introduction: The pathogeny of chronic rhinosinusitis with nasal polyposis (CRS/NP) has not been elucidated. Bacterial exotoxins have been implicated in many infl ammatory chronic diseases, such as chronic otitis, chronic tonsillitis, cholesteatomas, and more recently CRS/NP. We propose that the bacteria in CRS/NP are not only present in a planktonic state, but also occur in microbial communities as Biofilms. Objective: To determine and characterize the presence of Biofilms in CRS/NP. Methods: We performed a prospective study in 12 patients undergoing endoscopic sinus surgery for nasal polyposis. Ten patients without CRS/NP who underwent septoplasty were included as a control group. Tissue samples were obtained from the inferior turbinate mucosae. The bacteria were isolated and typifi ed and the material was examined in vitro using a spectrophotometer, and in vivo using optical microscopy and confocal scanning laser microscopy. Results: Moderate to high in vitro Biofilm-forming capacity was detected in 9 out of 12 patients with CRS/NP (mean [SD] optical density values of between 0.284 [0.017] and 3.337 [0.029]). The microorganisms isolated were Staphylococcus (5 patients), Streptococcus viridans, Pseudomonas aeruginosa, Enterococcus faecalis and Streptococcus viridans/Corynebacterium. Biofilms were demonstrated in vivo in 2 patients and no Biofilm structures were evident in any of the controls. Conclusion: This study demonstrates the presence of bacterial Biofilms in patients with CRS/NP. This chronic infl ammatory factor might contribute to nasal mucosa damage, increased infl ammatory cells in tissue, and the subsequent hyperplasic process.

Tipo de documento:	Artículo
Palabras clave:	Biofilms. Chronic rhinosinusitis. Confocal scanning laser microscopy. Nasal polyposis. Optical microscopy.
Temas:	R Medicina > R Medicina (General)
Unidad académica:	Universidad Católica de Córdoba > Facultad de Ciencias Químicas
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/4966

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