Disruption of antigenic variation is crucial for effective parasite vaccine

Rivero, Fernando David, Saura, Alicia ORCID: https://orcid.org/0000-0003-1537-506X, Prucca, César Germán ORCID: https://orcid.org/0000-0003-4165-4660, Carranza, Pedro Gabriel ORCID: https://orcid.org/0000-0002-1145-3852, Torri, Alessandro and Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 (2010) Disruption of antigenic variation is crucial for effective parasite vaccine. Nature Medicine, 16 (5). pp. 551-557. ISSN 1546170X

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Resumen

Giardia lamblia is a human intestinal pathogen. Like many protozoan microorganisms, Giardia undergoes antigenic variation, a mechanism assumed to allow parasites to evade the host's immune response, producing chronic and/or recurrent infections. Recently, we found that the mechanism controlling variant-specific surface protein (VSP) switching in Giardia involves components of the RNA interference machinery and that disruption of this pathway generates trophozoites simultaneously expressing many VSPs. Here we use these altered trophozoites to determine the role of antigenic variation in a gerbil model of giardiasis. Our results show that either primary infection with trophozoites simultaneously expressing many VSPs or immunization with purified VSPs from the transgenic cells protects gerbils from subsequent Giardia infections. These results constitute, to our knowledge, the first experimental evidence that antigenic variation is essential for parasite survival within hosts and that artificial disruption of this mechanism might be useful in generating vaccines against major pathogens that show similar behavior.

Tipo de documento:	Artículo
DOI:	https://doi.org/10.1038/nm.2141
Palabras clave:	Animals. Animals, Genetically Modified. Antigenic Variation. Gerbillinae. Giardia lamblia. Giardiasis. Humans. Intestines. Mice. Parasites. Protozoan Vaccines. RNA Interference
Temas:	R Medicina > R Medicina (General)
Unidad académica:	Universidad Católica de Córdoba > Facultad de Ciencias de la Salud
Google Académico:	Ver citaciones
URI:	http://pa.bibdigital.ucc.edu.ar/id/eprint/4987

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